| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 132 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | G9a/EHMT2 Rabbit Monoclonal Antibody detects endogenous levels of G9a/EHMT2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
G9a also known as NG36, EHMT2 is a methyltransferase that methylates lysine residues of histone H3. G9a plays a crucial role in the regulation of gene expression through its function as a histone methyltransferase, specifically targeting histone H3 lysine 9 for methylation. This post-translational modification is vital for the repression of developmental genes and is essential for early embryogenesis, highlighting G9a′s importance in cellular differentiation and development. G9a′s ability to add methyl groups to lysine residues not only influences transcriptional regulation but also contributes to the organization of chromatin structure, thereby impacting overall genomic stability. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293, Mouse kidney cells, using G9a/EHMT2 Rabbit Monoclonal Antibody (EAB21170) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 132 kDa; Observed band size: 180 kDa |
