| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | WB, IF/ICC | 55 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cleaved Caspase-8 (Asp374) Rabbit Polyclonal Antibody detects endogenous levels of Caspase-8 protein resulting from cleavage adjacent to Asp374. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Caspase-8 also known as MCH5, CASP8 is a member of the cysteine-aspartic acid protease (caspase) family. Caspase-8 plays a crucial role in the apoptotic signaling pathway, acting as an initiator caspase that activates downstream effector caspases through the cleavage of their inactive precursors. This process is vital for the regulation of programmed cell death, particularly in response to death receptor signaling via tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and Fas (TNFRSF6). Caspase-8 is primarily located in the cytoplasm and is recruited to the death-inducing signaling complex (DISC) upon binding to the adaptor molecule FADD, where activation occurs. The active form of caspase-8, a heterodimer composed of p18 and p10 subunits, is then released from the DISC to propagate the apoptotic signal. |
| Product Image Gallery | |
 Western blot analysis of extracts on THP-1 treated with TNFalpha, using Cleaved Caspase-8 (Asp374) Rabbit Monoclonal Antibody (EAB22374) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 55 kDa; Observed band size: 41, 43 kDa |
