| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 69 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | DLAT/PDC-E2 Rabbit Monoclonal Antibody detects endogenous levels of DLAT/PDC-E2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
DLAT also known as PDC-E2 plays a crucial role in the pyruvate dehydrogenase multienzyme complex, which is essential for converting pyruvate into acetyl-CoA, a key metabolic intermediate that fuels the citric acid cycle. This function is vital for energy production and metabolic regulation in cells, particularly in tissues with high energy demands such as the heart and brain. The presence of autoantibodies against DLAT is a hallmark of primary biliary cirrhosis, a chronic autoimmune liver disease characterized by the destruction of bile ducts, leading to cholestasis and liver damage. DLAT features a unique structure with both an amino-terminal lipoyl-bearing domain and a carboxy-terminal catalytic domain, which are critical for enzymatic activity and interaction with other proteins in the metabolic pathway. |
| Product Image Gallery | |
 Western blot analysis of extracts on Ramos, HT1080, C6 cells, using DLAT/PDC-E2 Rabbit Monoclonal Antibody (EAB22042) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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