| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 91 | Human, Rat, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:50-200 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MCM3 Mouse Monoclonal Antibody detects endogenous levels of MCM3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MCM3 is a member of the mini-chromosome maintenance (MCM) protein family. MCM3 plays a crucial role in the regulation of DNA replication, ensuring that DNA is replicated only once per cell cycle. MCM3 is located in the nucleus, where MCM3 forms part of the MCM2-7 complex, which acts as a helicase to unwind DNA during replication. The proper functioning of MCM3 and associated proteins is essential for the initiation of DNA synthesis, and any dysregulation can have significant implications for cell proliferation and tumorigenesis. MCM3 is also subject to post-translational modifications, which can influence MCM3 activity and interactions with other proteins, further underscoring MCM3′s importance in cellular processes. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, PC12, COS7, HepG2 cells, using MCM3 Mouse Monoclonal Antibody (EAB23003) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue, using MCM3 Mouse Monoclonal Antibody (EAB23003) at 1:200 dilution.
 Immunofluorescence analysis of Hela cell, using MCM3 Mouse Monoclonal Antibody (EAB23003)(green) at 1:200 dilution.
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