| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IF/ICC | 16 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | p16 INK4A Rabbit Monoclonal Antibody detects endogenous levels of p16 INK4A protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
p16 INK4A is also known as CDKN2A, MLM, ARF is often transcriptionally activated in cells undergoing senescence and is one of the main regulators of this program, and it is upregulated in multiple tissues during aging. p16 INK4A plays a crucial role as a negative regulator of the cell cycle, specifically inhibiting cyclin-dependent kinase 4 (Cdk4) activity, which is essential for the transition from the G1 phase to the S phase of the cell cycle. By binding to Cdk4, p16 INK4A prevents the formation of the active Cdk4/cyclin D complex, thereby halting cell proliferation and maintaining cellular integrity. This regulatory function is particularly important in the context of tumor suppression, as the gene encoding p16 INK4A is frequently altered in various cancers, with homozygous deletions and point mutations observed in established human tumor cell lines. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HEK293 cells, using p16 INK4A Rabbit Monoclonal Antibody (EAB22618) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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