| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, FC, ELISA | 91 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TOP1 Mouse Monoclonal Antibody detects endogenous levels of TOP1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TOP1 also known as DNA topoisomerase I, Topo I is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. TOP1 plays a crucial role in maintaining DNA structure integrity during cellular processes such as replication, transcription, and recombination. By introducing a transient break in one strand of the DNA helix, TOP1 alleviates torsional strain that arises ahead of the replication fork, preventing DNA supercoiling and ensuring proper DNA function. This enzymatic activity is vital for cellular health, as dysregulation of TOP1 can lead to genomic instability and has been implicated in various cancers, making TOP1 a significant target for anticancer therapies. TOP1 interacts with several other proteins, including transcription factors and repair proteins, which further underscores TOP1′s importance in cellular processes and highlights TOP1′s potential as a therapeutic target. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jukat, MCF7, HCT116 cells, using TOP1 Mouse Monoclonal Antibody (EAB21639) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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