| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 70 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Ku70 Rabbit Monoclonal Antibody detects endogenous levels of Ku70 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Ku70 also known as XRCC6 is primarily located in the nucleus, where Ku70 plays a vital role in recognizing and binding to double-strand breaks in DNA, thereby facilitating the recruitment of other repair factors. The importance of Ku70′s nuclear localization cannot be overstated, as Ku70 ensures ready availability to respond to DNA damage, which is crucial for preventing mutations and maintaining cellular integrity. Additionally, Ku70 interacts with its partner subunit, Ku86, to form a heterodimer that is integral to the non-homologous end joining (NHEJ) pathway of DNA repair. This pathway is particularly important in the context of cellular responses to genotoxic stress, making Ku70 a key player in the preservation of genetic information and the prevention of cancerous transformations. With diverse roles in DNA repair and transcriptional regulation, Ku70 is a significant target for research in cancer biology and therapeutic interventions. |
| Product Image Gallery | |
 Western blot analysis of extracts on Ramos cells, using Ku70 Rabbit Monoclonal Antibody (EAB22750) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
