| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 39/37 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-MKK3 (Ser189)/MKK6 (Ser207) Rabbit Monoclonal Antibody recognizes endogenous levels of MKK3 and MKK6 proteins only when phosphorylated at Ser189 (MKK3) or Ser207 (MKK6). |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MKK3/MKK6 is a protein that plays a crucial role in the MAPK signaling pathway, specifically in the activation of p38 MAP kinase and JNK MAP kinases, which are essential for cellular responses to stress and inflammation. MKK3/MKK6 recognizes a protein vital for regulating various physiological processes, including cell differentiation, proliferation, and apoptosis. Dysregulation of this pathway can lead to various diseases, including cancer and inflammatory disorders, making MKK3/MKK6 a significant target for therapeutic intervention. Understanding MKK3/MKK6 interactions with other proteins in the MAPK pathway can provide insights into regulatory mechanisms and potential roles in disease progression. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with TPA, using Phospho-MKK3 (Ser189)/MKK6 (Ser207) Rabbit Monoclonal Antibody (EAB22147) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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