| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2a | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 153 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | GTBP/MSH6 Mouse Monoclonal Antibody detects endogenous levels of GTBP/MSH6 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MSH6 also known as GTBP is a member of the DNA mismatch repair MutS family. MSH6 plays a crucial role in DNA mismatch repair pathway, which maintains genomic stability by correcting DNA replication errors. MSH6 is primarily located in the nucleus, where MSH6 forms a heterodimer with MSH2 to recognize and bind to G/T mismatches in DNA. This interaction initiates a cascade of events leading to excision and resynthesis of erroneous DNA segments. MSH6′s importance in this pathway is underscored by MSH6′s association with hereditary nonpolyposis colorectal cancer (HNPCC), where mutations in mismatch repair genes lead to microsatellite instability and increased cancer risk. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549, HepG2, MCF7, HEK293 cells, using GTBP/MSH6 Mouse Monoclonal Antibody (EAB21243) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 153 kDa; Observed band size: 160 kDa  Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, using GTBP/MSH6 Mouse Monoclonal Antibody (EAB21243) at 1:200 dilution.
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