| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, FC, ELISA | 47 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ASS1 Mouse Monoclonal Antibody detects endogenous levels of ASS1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ASS1(argininosuccinate synthase 1) plays a crucial role in the urea cycle, catalyzing the second-to-last step in the arginine biosynthetic pathway. This enzyme is essential for the detoxification of ammonia in the liver, converting citrulline and aspartate into argininosuccinate, which is subsequently cleaved to produce arginine and fumarate. A deficiency in ASS1 can lead to citrullinemia (CTLN1), an autosomal recessive disorder characterized by severe vomiting spells and cognitive impairments, highlighting the importance of ASS1 in metabolic health. The tetrameric structure of ASS1, composed of identical subunits, is vital for enzymatic function, as this composition allows for proper substrate binding and catalytic activity, making ASS1 protein interactions critical for understanding related metabolic disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on A431, Jurkat, A549, PC12 cells, using ASS1 Mouse Monoclonal Antibody (EAB21811) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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