| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 22 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ASC Rabbit Monoclonal Antibody detects endogenous levels of ASC protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ASC also known as TMS1, PYCARD is a critical member of the CARD-containing adaptor protein family. ASC playing a pivotal role in apoptotic signaling by mediating interactions between adaptor proteins like Apaf-1 and pro-caspases such as CASP9. Structurally, ASC comprises a Pyrin-like domain (PYD) at the N-terminus and an 87-residue caspase recruitment domain (CARD) at the C-terminus, which are essential for its function in apoptosis regulation. Upon induction of apoptosis, ASC undergoes a transformation from diffuse cytoplasmic distribution to forming punctate or spherical perinuclear aggregates, a process that can be visualized through fluorescence microscopy. This aggregation is significant in the cellular response to apoptotic stimuli, including treatments with retinoic acid and various anti-tumor drugs. |
| Product Image Gallery | |
 Western blot analysis of extracts on THP-1 cells, using ASC Rabbit Monoclonal Antibody (EAB22670) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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