| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 109 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | IRE1α Rabbit Monoclonal Antibody detects endogenous levels of IRE1α protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IRE1α plays a crucial role in the unfolded protein response (UPR), a cellular stress response activated when misfolded or unfolded proteins accumulate in the endoplasmic reticulum (ER). This response is vital for maintaining cellular homeostasis and preventing apoptosis under stress conditions. IRE1α, along with IRE1β homolog, is localized in the ER lumen and features a transmembrane domain spanning the ER membrane, as well as a cytosolic Ser/Thr kinase domain. Upon activation, IRE1α undergoes oligomerization and trans-phosphorylation, which enhances kinase activity and leads to UPR-targeted gene transcription. This process is essential for proper protein folding and ER function restoration. Additionally, IRE1α is involved in Jun N-Terminal Kinase (JNK) phosphorylation, thereby activating the cellular MAP kinase pathway, which further underscores IRE1α′s importance in cellular signaling and stress response mechanisms. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, LnCap cells, using IRE1α Rabbit Monoclonal Antibody (EAB22642) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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