| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 41 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Wnt1 Mouse Monoclonal Antibody detects endogenous levels of Wnt1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Wnt1 is a member of the highly conserved Wnt gene family. Wnt1 plays a crucial role in regulating cellular growth and differentiation, making Wnt1 vital for various developmental processes. Wnt1 is a cysteine-rich secreted glycoprotein that associates with cell membranes and is essential for normal development of the embryonic nervous system. Wnt1 expression is typically restricted to the embryonic neural tube and adult spermatids, highlighting Wnt1′s importance in developmental biology. Aberrant Wnt-1 expression in mammary tissue has been linked to hyperplasia and tumorigenic progression, underscoring Wnt1′s role in cancer biology. Additionally, Wnt1 interacts with β-catenin, a key player in cellular adhesion and a downstream target in the Wnt signaling pathway, which further emphasizes Wnt1′s significance in both normal and pathological processes. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, NIH/3T3 cells, using Wnt1 Mouse Monoclonal Antibody (EAB22933) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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