| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 60 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 IP=1:20-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CDT1 Rabbit Monoclonal Antibody detects endogenous levels of CDT1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CDT1 also known as DUP is involved in the formation of the pre-replication complex that is necessary for DNA replication. CDT1 is a nuclear-localizing replication initiation factor, specifically expressed during the G1 and S phases of the cell cycle. CDT1 facilitates the loading of the minichromosome maintenance (MCM) complex onto chromatin at the end of mitosis, a critical step necessary for DNA replication initiation. While CDT1 mRNA maintains consistent expression throughout the cell cycle, CDT1 levels decrease significantly after S-phase onset, indicating proteolytic degradation′s essential role in maintaining proper levels and preventing untimely DNA replication. CDT1 also interacts with DNA replication inhibitor geminin during S and G2 phases, preventing inappropriate origin firing. CDT1′s ability to reverse geminin′s inhibition of DNA replication in cell-free extracts highlights CDT1′s significance in DNA synthesis and cell cycle progression regulation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HepG2, Jurkat, SH-SY5Y cells, using CDT1 Rabbit Monoclonal Antibody (EAB22834) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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