| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 52 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PPARα Mouse Monoclonal Antibody detects endogenous levels of PPARα protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PPARα also known as PPARalpha, PPARA is a pivotal nuclear hormone receptor that plays a significant role in regulating fatty acid metabolism, lipid homeostasis, and overall energy balance, making PPARα essential for maintaining metabolic health. By forming heterodimers with retinoid X receptors (RXRs), PPARα binds to specific PPAR-response elements (PPREs) in DNA, thereby modulating the transcription of genes involved in lipid metabolism and energy utilization. PPARα functionality is crucial in primary hepatocytes, where PPARα governs the expression of proteins necessary for fatty acid oxidation and overall metabolic processes. Additionally, PPARα interacts with other nuclear receptors such as LXRα and thyroid receptors, which can act as antagonists to PPARα binding with RXRα at PPREs, highlighting the intricate regulatory mechanisms governing lipid metabolism. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, HSCT6 cells, using PPARα Mouse Monoclonal Antibody (EAB22127) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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