| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IF/ICC, FC | 26 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CD16 Rabbit Monoclonal Antibody detects endogenous levels of CD16 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CD16 is a crucial immune receptor that exists in two primary isoforms: CD16A (FcγRIIIA), a transmembrane protein expressed on natural killer (NK) cells and macrophages, and CD16B (FcγRIIIB), a glycosylphosphatidylinositol (GPI)-anchored protein found on neutrophils. The distinct structural configurations of these isoforms are essential for their specific roles in the immune response, with CD16A requiring association with the γ subunit of FcεRI or the ζ subunit of the TCR-CD3 complex for proper cell surface expression. This structural versatility allows CD16 to mediate antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis by binding the Fc region of IgG antibodies, thereby targeting and eliminating pathogens or infected cells effectively. CD16′s ability to exist both as a transmembrane and a GPI-anchored receptor highlights versatile functions in immune regulation and response. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, PC12, HepG2 cells, using CD16 Rabbit Monoclonal Antibody (EAB22416) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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