| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 82 | Human, Mouse, Rat, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CD44 Mouse Monoclonal Antibody detects endogenous levels of CD44 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CD44 also known as HCAM is a 742 amino acid single-pass type I membrane protein that plays a crucial role in cell adhesion and migration, functioning as a receptor for hyaluronic acid (HA) and interacting with ligands like osteopontin (OPN). This interaction is vital for mediating cell-cell and cell-matrix connections, which are essential during processes such as hematopoiesis, lymphocyte activation, and tumor metastasis. HCAM′s structure includes a Link domain and is subject to alternative splicing, resulting in multiple isoforms, including CD44R, CDw44, CD44S, CD44H (hematopoietic), and CD44E (epithelial). CD44H isoform shows high expression in cancer tissues, indicating significant involvement in tumor progression and highlighting HCAM′s importance in both normal physiological processes and pathological conditions. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, COS7, C6 cells, using CD44 Mouse Monoclonal Antibody (EAB21575) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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