| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, FC, ELISA | 81 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Daxx Mouse Monoclonal Antibody detects endogenous levels of Daxx protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Daxx is a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. Daxx plays a crucial role in the regulation of apoptosis, particularly in the context of the FAS-mediated apoptotic pathway. When the FAS receptor is activated by its ligand, activation triggers a cascade of events leading to programmed cell death, which is essential for maintaining immune system balance and tissue homeostasis. Daxx is a key player in this process, as Daxx interacts with the death domain of the FAS receptor, facilitating the activation of Jun N-terminal kinase (JNK) and promoting apoptosis. Notably, while Daxx does not possess a death domain, Daxx overexpression can enhance FAS-mediated apoptosis by activating the JNK pathway. This pathway operates alongside, yet distinctly from, the FAS-FADD-FLICE pathway, which is another critical route for apoptosis. The Daxx pathway is sensitive to the apoptotic inhibitor protein Bcl-2, highlighting unique regulatory mechanisms in cell death. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562 cells, using Daxx Mouse Monoclonal Antibody (EAB21647) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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