| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, FC, ELISA | 85 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | IKKα Mouse Monoclonal Antibody detects endogenous levels of IKKα protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IKKα also known as CHUK is a member of the serine/threonine protein kinase family. IKKα plays a pivotal role in activating the transcription factor NFκB, which regulates immune responses and inflammation. Located primarily in the cytoplasm, IKKα phosphorylates the inhibitory protein IκB on specific serine residues, leading to IκB degradation and subsequent NFκB release into the nucleus. This process enables NFκB to translocate and activate genes involved in immune and inflammatory responses. NFκB-inducing kinase (NIK) stimulates IκB phosphorylation by IKKα, serving as a key regulator in signaling pathways activated by tumor necrosis factor (TNF) and interleukin-1 (IL-1). The functional IKK complex, comprising IKKα, IKKβ, and IKKγ (NEMO), is essential for IκB phosphorylation, highlighting IKKα′s role in maintaining cellular homeostasis and responding to inflammatory stimuli. |
| Product Image Gallery | |
 Western blot analysis of extracts on Raji, Jurkat, THP-1, K562 cells, using IKKα Mouse Monoclonal Antibody (EAB21683) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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