| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 37 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Mcl-1 Mouse Monoclonal Antibody detects endogenous levels of Mcl-1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Mcl-1 also known as Myeloid cell leukemia-1, Mcl1, Gm52627 is a critical anti-apoptotic protein within the B-cell lymphoma 2 (Bcl-2) family that plays a vital role in regulating cell survival by inhibiting apoptosis through interactions with pro-apoptotic proteins like Bak and Bax, thereby preventing mitochondrial outer membrane permeabilization and cytochrome c release, essential steps in the intrinsic apoptotic pathway. Mcl-1 functions to ensure normal development, tissue homeostasis, and immune system function, particularly in the maintenance of B and T lymphocyte populations. Dysregulation or overexpression of Mcl-1 can lead to the survival of abnormal cells and has been implicated in the development of various cancers, including leukemia, lymphoma, and solid tumors, making Mcl-1 an important target for cancer research and therapeutic intervention. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, HL60 cells, using Mcl-1 Mouse Monoclonal Antibody (EAB21283) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB2100Q) at 1:5000 dilution.
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