| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 115 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MYPT1 Rabbit Monoclonal Antibody detects endogenous levels of MYPT1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MYPT1 also known as PPP1R12A is one of the subunits of myosin phosphatase. MYPT1 plays a crucial role in regulating the interaction between actin and myosin, which is essential for muscle contraction and cellular movement. MYPT1 is primarily localized on stress fibers, where MYPT1 is strategically positioned near the cell membrane and at cell-cell contacts, allowing MYPT1 to effectively modulate myosin phosphatase activity in response to various signaling pathways. MYPT1 is regulated by phosphorylation, particularly at Tyrosine 654 by myotonic dystrophy protein kinase, which influences myosin light chain phosphorylation and smooth muscle contraction. MYPT1′s phosphorylation state is critical, as MYPT1 determines the sensitivity of smooth muscle to calcium, thereby impacting vascular tone and blood pressure regulation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Daudi cells, using MYPT1 Rabbit Monoclonal Antibody (EAB21892) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 115 kDa; Observed band size: 140 kDa |
