| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 36 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PBK Mouse Monoclonal Antibody detects endogenous levels of PBK protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PBK(PDZ-binding kinase) also known as SPK is a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. PBK plays a crucial role in regulating cellular processes, particularly in cell cycle control and cellular proliferation. PBK is predominantly expressed in the placenta and is notably absent in adult brain tissue, highlighting specific functional relevance in reproductive biology. PBK interaction with PDZ domain-containing proteins, such as tumor suppressor protein Dlg, underscores importance in cellular signaling pathways. PBK is activated by cell cycle checkpoint kinase Cdc2/cyclin B, which phosphorylates PBK, thereby influencing activity and potential oncogenic effects. PBK ability to modulate phosphorylation states of oncoproteins and tumor suppressor proteins makes this target significant for research in cancer biology and therapeutic development. |
| Product Image Gallery | |
 Western blot analysis of extracts on A431 cells, using PBK Mouse Monoclonal Antibody (EAB22413) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, using PBK Mouse Monoclonal Antibody (EAB22413) at 1:200 dilution.
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