| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC, FC | 63 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Shc Rabbit Monoclonal Antibody detects endogenous levels of Shc protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Shc also known as SHCA, SHC1 is a critical adapter protein that plays a pivotal role in signaling pathways activated by receptor tyrosine kinases, which are essential for various cellular responses, including growth and differentiation. Located primarily in the cytoplasm, Shc acts as a molecular bridge, linking activated receptors to downstream signaling molecules, thereby facilitating signal transmission to the nucleus. This function allows cells to respond appropriately to external growth signals, ensuring proper cellular function and homeostasis. Notably, Shc interacts with other key proteins, such as GRB2 and Sos, to activate the Ras signaling pathway, which is vital for cell proliferation and survival. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, MCF7 cells, using Shc Rabbit Monoclonal Antibody (EAB21252) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 63 kDa; Observed band size: 46,52,66 kDa |
