| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 73 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-c-Raf (Ser43) Rabbit Polyclonal Antibody detects endogenous levels of c-Raf protein only when phosphorylated at Ser43. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
c-Raf also known as Raf-1 is a crucial serine/threonine protein kinase that plays a significant role in the regulation of cell signaling pathways, particularly in cell growth and differentiation. Located primarily in the cytoplasm, c-Raf is activated by various receptor tyrosine kinases and is essential for transmitting signals from the cell membrane to downstream effectors, including the MAPK/ERK pathway. This pathway is vital for cellular responses to growth factors and mitogenic signals, making c-Raf a key player in processes such as proliferation and survival. c-Raf activity is tightly regulated by its N-terminal domain, which prevents unregulated kinase activity under normal conditions. However, aberrations in c-Raf signaling can lead to oncogenic transformations, highlighting c-Raf ′s importance in cancer biology. |
| Product Image Gallery | |
 Western blot analysis of extracts on NIH/3T3 cells, using Phospho-c-Raf (Ser43) Rabbit Polyclonal Antibody (EAB14850) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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