| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 34 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CrkL Rabbit Monoclonal Antibody detects endogenous levels of CrkL protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CrkL plays a crucial role in cellular signaling pathways, particularly in regulating cell growth, differentiation, and survival, making CrkL an important target for understanding oncogenic processes. Located in the cytoplasm, CrkL interacts with various signaling molecules, facilitating signal transmission from cell surface receptors to downstream effectors, which is vital for maintaining normal cellular functions and responding to external stimuli. CrkL protein is characterized by one SH2 domain and two SH3 domains, allowing binding to phosphorylated tyrosine residues and mediating protein-protein interactions. This structural configuration is essential for signal transduction, as CrkL functions as an adaptor protein linking different signaling pathways, thereby influencing cellular responses to growth factors and other stimuli. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, K562, DU145 cells, using CrkL Rabbit Monoclonal Antibody (EAB22184) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 34 kDa; Observed band size: 39 kDa |
