| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 100 | Human, Mouse, Rat, Monkey, Rabbit |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | c-Cbl Mouse Monoclonal Antibody detects endogenous levels of c-Cbl protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
c-Cbl also known as CBL plays a crucial role in cellular signaling pathways by functioning as an E3 ubiquitin ligase, which is essential for the regulation of protein degradation and cellular homeostasis. Cbl′s function lies in modulating the activity of various signaling proteins, thereby influencing processes such as cell proliferation, differentiation, and survival. Dysregulation of Cbl has been implicated in several hematological malignancies, as Cbl is involved in the ubiquitination and subsequent degradation of proteins that promote oncogenic signaling. c-Cbl gene product is predominantly localized in the cytoplasm, where c-Cbl interacts with multiple signaling molecules, highlighting c-Cbl′s significance in maintaining normal cellular functions and preventing tumorigenesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Raji, K562, SKBR3, Raw264.7, PC12 cells, using c-Cbl Mouse Monoclonal Antibody (EAB22471) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 100 kDa; Observed band size: 120 kDa  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue, using c-Cbl Mouse Monoclonal Antibody (EAB22471) at 1:200 dilution.
 Immunofluorescence analysis of Hela cell, using c-Cbl Mouse Monoclonal Antibody (EAB22471) at 1:200 dilution.
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