| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 39 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TTF-1 Rabbit Monoclonal Antibody detects endogenous levels of TTF-1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TTF-1 also known as thyroid transcription factor-1, NKX2.1, NKX2-1 is a crucial member of the Nkx2 family of homeodomain-containing transcription factors, playing a vital role in regulating the transcriptional activity of thyroid-specific genes. TTF-1 is predominantly located in the epithelial cells of the lung, thyroid, and brain, where TTF-1 is essential for organogenesis and the maintenance of differentiated phenotypes. Specifically, TTF-1 is instrumental in lung morphogenesis as TTF-1 regulates the transcription of surfactant proteins A, B, and C, which are critical for proper lung function and gas exchange. Additionally, in the thyroid, TTF-1 enhances the expression of key thyroid-specific markers such as thyroglobulin, thyroperoxidase, and thyrotropin receptors, thereby influencing thyroid hormone synthesis and regulation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, Mouse lung cells, using TTF-1 Rabbit Monoclonal Antibody (EAB22212) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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