| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 124 | Human, Mouse, Rat, Rabbit |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:500-2000 IF/ICC=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Vinculin Mouse Monoclonal Antibody detects endogenous levels of Vinculin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin plays a crucial role in cellular adhesion and mechanotransduction, functioning as a key component of focal adhesions, which are specialized structures that connect the cytoskeleton to the extracellular matrix. These focal adhesions are essential for maintaining cell shape, enabling cell migration, and facilitating communication between the cell and its environment. Vinculin′s ability to interact with other cytoskeletal proteins, such as Talin and α-actinin, underscores vinculin′s importance in linking the actin cytoskeleton to integrins in the plasma membrane, thereby influencing cellular responses to mechanical stimuli. |
| Product Image Gallery | |
 Western blot analysis of extracts on HuvEc, K562, Mouse heart, Rat heart cells, using Vinculin Mouse Monoclonal Antibody (EAB21173) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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