| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 238 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | NumA Rabbit Monoclonal Antibody detects endogenous levels of NumA protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
NumA also known as NumA1 plays a critical role in organizing and stabilizing spindle poles during mitosis, which is essential for accurate chromosome segregation. Mapping to chromosome 11q13, NuMA gene encodes a noncentrosomal protein that binds to microtubules through its carboxyl terminal region, facilitating bipolar spindle formation by aggregating microtubules and interacting with motor proteins. This function is vital for maintaining genomic stability, as errors in spindle assembly can lead to aneuploidy and contribute to tumorigenesis. NuMA protein is predominantly localized to the spindle apparatus during mitosis, yet also appears in the nucleus and cytoplasm of post-mitotic neurons, indicating diverse roles in different cell types. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, SH-SY5Y cells, using NumA Rabbit Monoclonal Antibody (EAB21764) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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