| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IF/ICC, ELISA | 41 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | c-Fos Rabbit Monoclonal Antibody detects endogenous levels of c-Fos protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
c-Fos is a nuclear phosphoprotein that acts as a transcriptional regulator, characterized by rapid and transient expression in response to a wide array of stimuli, including growth factors, cytokines, and stress signals. c-Fos forms heterodimers with members of the Jun family—such as c-Jun, Jun B, and Jun D, to create the Activator Protein-1 (AP-1) transcription factor complex, which binds to specific DNA elements to regulate gene expression linked to cell proliferation, differentiation, and survival. Beyond interactions with Jun proteins, c-Fos also associates with transcription factors like ATF-2 and CREB, forming leucine zipper dimers that exhibit differential specificity for AP-1 and CRE sites. c-Fos is regulated by coactivators such as CBP/p300, which modulate activity through acetylation, influencing gene transcription outcomes. c-Fos is involved in regulating cytoskeletal organization and cellular motility, highlighting diverse functions beyond transcriptional regulation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF cells, using c-Fos Rabbit Monoclonal Antibody (EAB22122) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 41 kDa; Observed band size: 55 kDa |
