| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, FC | 68 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 IP=1:20-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PKCζ Rabbit Monoclonal Antibody detects endogenous levels of PKCζ protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PKCζ also known as PKC zeta, PRKCZ is an atypical member of the protein kinase C family, playing a crucial role in various cellular processes such as cell growth, differentiation, survival, gene expression, and membrane function. Unlike conventional PKCs, PKCζ activation is independent of calcium and diacylglycerol (DAG), allowing unique function within signaling pathways. PKCζ is involved in the regulation of NF-κB activation, insulin signaling, cell polarity, and the PI3K/Akt pathway, making PKCζ significant in understanding diseases like diabetes, cancer, and immune disorders. PKCζ′s involvement in cell survival and proliferation underscores PKCζ′s importance as a potential therapeutic target. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, HepG2 cells, using PKCζ Rabbit Monoclonal Antibody (EAB22648) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 68 kDa; Observed band size: 77 kDa |
