| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 77 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:100-500 IF/ICC=1:200-1000 FC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PKCα Mouse Monoclonal Antibody detects endogenous levels of PKCα protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PKCα also PRKCA, PKC alpha is a member of the protein kinase C (PKC) family. PKCα plays a crucial role in regulating numerous cellular functions such as cell growth, differentiation, gene expression, and hormone secretion, making PKCα vital for maintaining cellular homeostasis. PKCα is primarily located in the cytoplasm and translocates to the cell membrane upon activation, which is essential for PKCα′s role in signal transduction pathways. This translocation is triggered by diacylglycerols (DAG) and tumor-promoting phorbol esters, which bind to and activate PKCα, allowing PKCα to phosphorylate target proteins and modulate various cellular responses. The diverse expression patterns of PKC isoforms across different tissues highlight PKCα′s importance in specific signaling pathways, and PKCα′s unique cofactor dependencies further underscore PKCα′s distinct regulatory mechanisms within the PKC family. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HEK293, NIH/3T3 cells, using PKCα Mouse Monoclonal Antibody (EAB22325) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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