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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IF/ICC | 54 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IHC-P=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-YAP (Ser127) Rabbit Monoclonal Antibody detects endogenous levels of YAP protein only when phosphorylated at Ser127. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
YAP also known YAP1 is a pivotal component of the Hippo signaling pathway, functioning as a transcriptional co-activator that regulates essential cellular processes including cell proliferation, apoptosis, and organ size. The localization of YAP within the cell, whether in the nucleus or cytoplasm, is crucial for controlling gene expression and maintaining cellular homeostasis. By interacting with 14-3-3 proteins, YAP′s activity and subcellular distribution are tightly regulated, linking extracellular signals to nuclear responses that influence cell differentiation, cell cycle progression, and programmed cell death. This regulation is vital for normal physiological functions and is implicated in the development of various cancers when dysregulated. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with Calyculin A, using Phospho-YAP (Ser127) Rabbit Monoclonal Antibody (EAB21735) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 54 kDa; Observed band size: 54-75 kDa |
