| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 60 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Smad4 Mouse Monoclonal Antibody detects endogenous levels of Smad4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Smad4 also known as DPC4 is a member of the Smad family of signal transduction proteins. Smad4 plays a crucial role as a central mediator in the transforming growth factor-beta (TGFβ) and bone morphogenetic protein (BMP) signaling pathways, which are essential for regulating cellular processes such as proliferation, differentiation, and apoptosis. By interacting with other Smad family members, Smad4 facilitates transcriptional regulation of target genes, thereby influencing developmental processes and tissue homeostasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on A431, K562, HepG2 cells, using Smad4 Mouse Monoclonal Antibody (EAB22357) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue,
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