| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P | 32 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Caspase-3 Rabbit Polyclonal Antibody detects endogenous levels of Caspase-3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Caspase-3 is a crucial aspartate-specific cysteine protease within the ICE subfamily, characterized by its structure as an inactive proenzyme that undergoes proteolytic cleavage to form an active heterodimer composed of p17 and p11 subunits. This structural transformation is essential for its role in apoptosis, where the active enzyme systematically dismantles cellular components by cleaving key substrates like PARP, thereby ensuring orderly cell death. The precise arrangement of the p17 and p11 subunits within caspase-3′s structure is vital for enzymatic activity and interaction with other proteins in the apoptotic cascade, including the activation of caspase-6, -7, and -9. Understanding the structural integrity of caspase-3 is fundamental for elucidating function in maintaining cellular homeostasis, eliminating damaged or unnecessary cells, and regulating processes such as development, immune response, and cancer prevention. Caspase-3′s structural domains facilitate localization and interaction within the cytoplasm, making caspase-3 a key player in various physiological and pathological contexts. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells, using Caspase-3 Rabbit Polyclonal Antibody (EAB11066) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using Caspase-3 Rabbit Polyclonal Antibody (EAB11066) at 1:400 dilution.
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