| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2a | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 92 | Human, Mouse, Rat, Pig |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:500-2000 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Sortilin Mouse Monoclonal Antibody detects endogenous levels of Sortilin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Sortilin also known as sortilin-1, Gp95, NRT3 is a member of the VPS10-related sortilin family of proteins. Sortilin plays a crucial role in the central nervous system and peripheral tissues by mediating neurotensin signaling, which is essential for various physiological processes including pain modulation, thermoregulation, and metabolic control. Sortilin is primarily located in the endoplasmic reticulum and Golgi apparatus, where Sortilin undergoes post-translational modifications such as glycosylation, which are vital for proper folding, stability, and receptor activity. These modifications not only influence Sortilin ′s trafficking and localization but also Sortilin ′s interaction with neurotensin and other signaling molecules, thereby impacting downstream signaling pathways that regulate inflammatory responses and neuronal function. |
| Product Image Gallery | |
 Western blot analysis of extracts on SH-SY5Y, Mouse brain, Rat brain cells, using Sortilin Mouse Monoclonal Antibody (EAB22763) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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