| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P | 63 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | AATF Mouse Monoclonal Antibody detects endogenous levels of AATF protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
AATF(Apoptosis-antagonizing transcription factor) also known as Che-1 is a widely expressed nuclear protein that belongs to the AATF family. Hyperphosphorylated during the G1/S phase transition, AATF may function as a general inhibitor of the histone deacetylase HDAC1. AATF binding to the pocket region of Rb may displace HDAC1 from Rb/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Displacement of HDAC1 from Sp1 bound to the p21 promoter leads to increased expression of AATF. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, HepG2, K562 cells, using AATF Mouse Monoclonal Antibody (EAB22121) at 1:5000 dilution. Secondary antibody Goat Anti-RMouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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