| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 17 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | eIF5A Mouse Monoclonal Antibody detects endogenous levels of eIF5A protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
eIF5A also designated eIF-4D, eIF5A1, maps to chromosome 17p131 and encodes a 154 amino acid protein that is linked to cellular polyamine homeostasis. eIF5A1 localizes to the nuclear and cytoplasmic compartments of mammalian cells where it can stimulate ribosomal peptidyl-transferase and may be involved in nucleocytoplasmic mRNA transport and/or protein translation. eIF5A1 contains a unique spermidine-derived post-translational modification at Lys-50, hypusine, which is necessary for eIF5A1′s biochemical activity and for cellular proliferative signaling. |
| Product Image Gallery | |
 Western blot analysis of extracts on Raji cells, using eIF5A Mouse Monoclonal Antibody (EAB21625) at 1:1000 dilution. Secondary antibody Goat Anti-Mosue IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, using eIF5A Mouse Monoclonal Antibody (EAB21625) at 1:200 dilution.
 Immunofluorescence analysis of Hela cell, using eIF5A Mouse Monoclonal Antibody (EAB21625) at 1:200 dilution.
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