| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2a | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 48 | Human, Mouse, Rat, Rabbit |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Calreticulin Mouse Monoclonal Antibody detects endogenous levels of Calreticulin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Calreticulin also known as CALR is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, A549, MCF7 cells, using Calreticulin Mouse Monoclonal Antibody (EAB22495) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 48 kDa; Observed band size: 55 kDa  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue, using Calreticulin Mouse Monoclonal Antibody (EAB22495) at 1:200 dilution.
 Immunohistochemical analysis of paraffin-embedded Rat kidney tissue, using Calreticulin Mouse Monoclonal Antibody (EAB22495) at 1:200 dilution.
 Immunofluorescence analysis of NIH/3T3 cell, using Calreticulin Mouse Monoclonal Antibody (EAB22495)(green) at 1:100 dilution.
 Flow cytometric analysis of NIH/3T3 cell, using Calreticulin Mouse Monoclonal Antibody (EAB22495)(green) at 1:50 dilution.
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