| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 47 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ENO1/Enolase-1 Rabbit Polyclonal Antibody detects endogenous levels of ENO1/Enolase-1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Enolase-1 also known as α Enolase, ENO1 is a member of the enolase family. Enolase-1 plays a crucial role in the glycolytic pathway, facilitating the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is a key step in energy production. This enzymatic function is vital for cellular metabolism, particularly in tissues with high energy demands, such as muscle and brain. Notably, Enolase-1 is not only involved in glycolysis but also has been implicated in various cellular processes, including differentiation and stress responses. Enolase-1 expression across a wide range of tissues underscores its importance in maintaining metabolic homeostasis, and expression levels can serve as a biomarker for certain pathological conditions. With an ability to form both homodimers and heterodimers with other enolase isoforms, Enolase-1′s structural versatility further enhances its functional significance in cellular metabolism. |
| Product Image Gallery | |
 Western blot analysis of extracts on PC12 cells, using ENO1/Enolase-1 Rabbit Polyclonal Antibody (EAB11506) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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