| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IP, IHC-P, IF, ChIP, ELISA | 152 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF=1:100-500 ChIP=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Brd4 Mouse Monoclonal Antibody detects endogenous levels of Brd4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Brd4 is a member of the bromodomain and extraterminal (BET) family, characterized by two bromodomains that facilitate interaction with acetylated lysines on histones and non-histone proteins. This interaction regulates gene expression, particularly in cell cycle progression and transcriptional activation. Brd4 plays a significant role in assembling transcriptional machinery at target gene promoters, essential for proper cell growth and proliferation. Dysregulation of Brd4 has been implicated in various cancers, where aberrant function can lead to uncontrolled cell division and tumor progression. Understanding Brd4′s role in cellular processes highlights both normal physiological importance and potential as a therapeutic target in cancer treatment. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, Jurkat, HEK293 cells, using Brd4 Mouse Monoclonal Antibody (EAB22485) at 1:3000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 152 kDa; Observed band size: 200 kDa |
