| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 159 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-BLM (Thr99) Rabbit Polyclonal Antibody detects endogenous levels of BLM protein only when phosphorylated at Thr99. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
BLM(Bloom syndrome) is an autosomal recessive disorder characterized by growth deficiency, microcephaly and immunodeficiency among others. BLM plays a crucial role in maintaining genomic stability by unwinding DNA during replication and repair processes, which is vital for preventing mutations that can lead to cancer. BLM is primarily located in the nucleus, where BLM interacts with both single- and double-stranded DNA, and BLM′s ATPase activity is essential for its helicase function. Mutations in the BLM gene result in Bloom′s syndrome, characterized by an increased frequency of sister chromatid exchange, which is a hallmark of genomic instability. This instability is linked to a higher predisposition to various cancers, making BLM a significant focus of research in cancer biology and genetics. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2 cells, using Phospho-BLM (Thr99) Rabbit Polyclonal Antibody (EAB11407) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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