| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 128 | Human, Mouse, Rat, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HAUSP/USP7 Mouse Monoclonal Antibody detects endogenous levels of HAUSP/USP7 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HAUSP also known as USP7 belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. HAUSP plays a critical role in regulating the stability of tumor suppressor protein p53 by deubiquitinating p53, which is essential for maintaining cellular homeostasis and preventing tumorigenesis. HAUSP is predominantly localized in the nucleus, where HAUSP associates with nuclear structures known as ND10, which are involved in various cellular processes such as stress response, oncogenesis, and viral infection. HAUSP binding to ND10 is significant because this interaction highlights HAUSP′s role in modulating cellular responses to stress and potential implications in cancer biology. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HepG2, Jurkat, HCT116, SW480, HT29 cells, using HAUSP/USP7 Mouse Monoclonal Antibody (EAB22499) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue, using HAUSP/USP7 Mouse Monoclonal Antibody (EAB22499) at 1:200 dilution.
 Immunofluorescence analysis of Hela cell, using HAUSP/USP7 Mouse Monoclonal Antibody (EAB22499)(green) at 1:200 dilution.
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