| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 33 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PHB2 Mouse Monoclonal Antibody detects endogenous levels of PHB2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PHB2 also known as Prohibitin 2 is an evolutionarily conserved protein that plays a crucial role in regulating the cell cycle, particularly by inhibiting the G1 to S phase transition, which is vital for maintaining proper cellular proliferation and preventing uncontrolled growth. PHB2 is predominantly localized in the inner mitochondrial membrane, where PHB2 not only contributes to mitochondrial function but also acts as a tumor suppressor by interacting with retinoblastoma (Rb) protein and inhibiting E2F family members, thereby regulating gene expression in response to various signaling pathways. PHB2 is involved in B cell maturation at the plasma membrane, highlighting Prohibitin 2′s importance in immune responses. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HEK293, Jurkat, 4T1, MCF7, NIH/3T3 cells, using PHB2 Mouse Monoclonal Antibody (EAB22397) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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