| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 62 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Neurofilament-L/NF-L Mouse Monoclonal Antibody detects endogenous levels of Neurofilament-L/NF-L protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
NF-L(Neurofilament-L) also known as NEFL is a crucial component of the neuronal cytoskeleton. NF-L plays a significant role in maintaining the structural integrity of neurons. Located primarily in the axons of neurons, NF-L contributes to the regulation of axon caliber, which is essential for proper nerve signal conduction. The dynamic nature of neurofilaments allows them to interact with other cytoskeletal elements, such as microtubules and actin filaments, thereby influencing overall neuronal function and stability. Importantly, alterations in NF-L phosphorylation and metabolism are often associated with neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease, making NF-L a valuable biomarker for studying these conditions. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Mouse brain cells, using Neurofilament-L/NF-L Mouse Monoclonal Antibody (EAB11349) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue, using Neurofilament-L/NF-L Mouse Monoclonal Antibody (EAB11349) at 1:200 dilution.
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