| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 113 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | AF-10 Rabbit Polyclonal Antibody detects endogenous levels of AF-10 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
AF-10 also knwon as MLLT10 is a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. AF-10 plays a significant role in gene expression regulation, particularly in acute myeloid leukemia, where AF-10 is implicated in the t(10;11) chromosomal translocation. This translocation leads to fusion proteins that can disrupt normal cellular functions, making AF-10 a key player in oncogenesis. Human AF-10 protein consists of 1027 amino acids and shares 90% identity with mouse AF-10, which contains 1061 amino acids. AF-10 is predominantly expressed in testis, highlighting AF-10′s potential role in reproductive biology and development. |
| Product Image Gallery | |
 Western blot analysis of extracts on COLO205, Jurkat cells, using AF-10 Rabbit Polyclonal Antibody (EAB11317) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue, using MLLT10 Rabbit Polyclonal Antibody (EAB11317) at 1:200 dilution.
 Immunofluorescence analysis of Hela cell, using MLLT10 Rabbit Polyclonal Antibody (EAB11317)(red) at 1:200 dilution.
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