| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 29 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:200-1000 IF/ICC=1:200-1000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PGAM1 Mouse Monoclonal Antibody detects endogenous levels of PGAM1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PGAM1 is a member of the phosphoglycerate mutase family. PGAM1 plays a crucial role in cellular metabolism, specifically in the interconversion of 3-phosphoglycerate and 2-phosphoglycerate, which are key intermediates in glycolysis and gluconeogenesis. This enzymatic activity is vital for maintaining energy homeostasis and supporting cellular functions, particularly in tissues with high metabolic demands. PGAM1, along with its isozyme PGAM2, can form homodimers or heterodimers, with PGAM1 predominantly expressed in various tissues while PGAM2 is primarily found in skeletal muscle, mature sperm cells, and the heart. The unique dimerization of these proteins allows for fine-tuning of metabolic pathways, making PGAM1 an important target for studying metabolic disorders and potential therapeutic interventions. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, HepG2, NIH/3T3 cells, using PGAM1 Mouse Monoclonal Antibody (EAB22577) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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