| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 47 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | NSE Mouse Monoclonal Antibody detects endogenous levels of NSE protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
NSE also known as γ Enolase, ENO2 is one of three isoenzymes of enolase, which are highly conserved cytoplasmic glycolytic enzymes crucial for energy metabolism. NSE plays a crucial role in the glycolytic pathway, specifically in the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is a key step in energy production. NSE is predominantly expressed in the nervous system, making gamma Enolase (NSE-P1) antibody an important marker for neuronal differentiation and function. NSE presence in neural tissues, as opposed to α Enolase and β Enolase isoforms found in various tissues and muscle respectively, highlights NSE′s significance in neurological studies and potential implications in neurodegenerative diseases. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, 293T cells, using NSE Mouse Monoclonal Antibody (EAB11299) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, using NSE Mouse Monoclonal Antibody (EAB11299) at 1:200 dilution.
 Immunofluorescence analysis of Hela cell, using NSE Mouse Monoclonal Antibody (EAB11299)(green) at 1:200 dilution.
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