| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IP, IHC-P, IF/ICC | 44 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:20-200 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Erk1 (Tyr204) Rabbit Polyclonal Antibody detects endogenous levels of Erk1 protein only when phosphorylated at Tyr204. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Erk1(Extracellular signal-regulated kinase 1) also known as ERT2, p44, MAPK3 is a member of the MAP kinase family. ERK1 plays a pivotal role in the MAPK/ERK signaling pathway, which transmits extracellular signals into intracellular responses. The primary function of ERK1 is to regulate essential cellular processes such as proliferation, differentiation, survival, and development. Activation of ERK1 occurs through dual phosphorylation by upstream kinases MEK1 and MEK2 in response to stimuli like growth factors, cytokines, and mitogens. Once activated, ERK1 translocates to the nucleus where ERK1 phosphorylates a variety of substrates, including transcription factors such as ELK1, c-Fos, and c-Myc, leading to the regulation of genes involved in cell cycle progression and differentiation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells treated with PMA, using Phospho-Erk1 (Tyr204) Rabbit Polyclonal Antibody (EAB14923) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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