| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC, FC | 227 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CHD3 Rabbit Monoclonal Antibody detects endogenous levels of CHD3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CHD3 also also known as Mi2-α is a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD3 plays a crucial role in chromatin remodeling, which is essential for the regulation of gene expression. CHD3 is a component of the mSin3 corepressor complex, which is involved in the deacetylation of histones, thereby influencing transcriptional silencing. The interaction of CHD3 with histone deacetylases, such as HDAC1 and HDAC2, highlights its significance in maintaining chromatin structure and function. By facilitating the removal of acetyl groups from lysine residues on histones, CHD3 contributes to the compaction of chromatin, making DNA less accessible to transcription factors and ultimately regulating gene expression. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, SH-SY5Y, MCF7 cells, using CHD3 Rabbit Monoclonal Antibody (EAB21527) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
